In his early youth Ondřej Holub went through Kühn’s children’s choir and the Pueri gaudentes boys’ choir, thanks to which he became a child soloist of the Prague State Opera in a production of Mozart’s The Magic Flute. He studied classical singing privately with Prof. Lenka Pištěcká, while studying at the Prague Conservatory with Prof. Valentin Prolat and later developed his singing skills with Prof. Jiří Kotouč.
Since 2019 he has been collaborating with the internationally renowned Collegium 1704, where he performs as a soloist as a soloist and ensemble singer, he also regularly collaborates with Ensemble Damian, a historically informed interpretation of Baroque opera. As a soloist he regularly collaborates with a number of ensembles and orchestras (Prague Symphony Orchestra FOK, Hradec Králové Philharmonic, South Bohemian Chamber Philharmonic, etc. He is also an ensemble singer with most of the leading domestic early music ensembles – Schola Gregoriana Pragensis, Collegium Marianum, Cappella Mariana, Ensemble Inégal, Czech Ensemble Baroque, Victoria Ensemble, Musica Florea, with whom he regularly participates in prestigious domestic and international festivals.
From 2013-2019 he was a member of the vocal ensemble Martinů Voices, which ranks among the elite performers of 20th and 21st century music. In 2007, he founded the male vocal quintet Rudolfvoice with former members of the Pueri Gaudentes choir, with whom he performed at the Kennedy Center in Washington, DC. He also occasionally collaborates with the Prague Philharmonic Choir, with whom he performed Gustav Mahler’s Symphony No. 2 at New York’s Carnegie Hall on the centenary of the founding of Czechoslovakia.